Ms. sign

This enemy appears in almost any level when the player takes too much time, normally when the player's timed score reaches 0. She is not a threat and is mostly just there to signify that you are taking too long, however she still does attack, dealing one damage per hit. In some levels, there are different conditions for when and how Ms. Sign spawns, and in an even smaller selection of levels, they indicate Assassin Bears that are about to be spawned.

In Last Gang, Ms. Sign may spawn earlier than usual (2 mins 6.6s), along with Assassin Bears.

Specifically in Chubby B. Goode, Ms. Sign spawns twice (first at 10 mins, second one at 14 mins 26.6s), and after that the player will end up facing highly magnified Assassin Bears afterwards.

Strangely, in Learned to Love, Ms. Signs are completely replaced by Assassin Bears.

In Gouache Ghouls, Ms. Sign spawns infinitely after 66.6 secs, taking up the enemy limit. If you hit the base before that happens, extremely buffed Angels will appear and destroy the player's base.

However in some levels she can be used as a cheese method. For example, the level Dark Souls (Insane) can be cheesed by waiting 15 minutes for her to spawn over and over until there are 10 of them since there are no more enemy's allowed out than 10.

10,000 health 1 damage